## HIGHLY REPRODUCIBLE SHORT READ SEQUENCING

DNA EF Library Prep (powered by Watchmaker)™ offers an automated, streamlined process for efficiently converting DNA of variable quantities into Illumina™ compatible sequencing ready libraries. The process includes enzymatic fragmentation, end-repair, A-tailing, adapter ligation and bead-based size selection, producing libraries with minimal bias and artifacts. The App supports PCR free WGS across multiple sequencing platforms, whole exome sequencing, and targeted sequencing by offering different library fragment sizes.

Compatible sequencing platforms: Illumina Sequencers, UG 100, Element AVITI

### key benefits

- **Highly consistent fragment size distributions**  
  **‍** Delivers uniform fragment lengths run after run, improving library quality and downstream sequencing performance.

- **Exceptionally low duplication rates (<10%)**  
  **‍** Maximizes usable reads and sequencing efficiency, even in demanding WGS applications.

- **Proven, production-ready reliability**  
  **‍** Fully automated walk-away workflow with a failure rate of **<1 in 300 runs**, enabling confident scale-up in high-throughput labs.

## App Specifications

**Input:** 100-500 ng DNA  
**Output:** Sequencing ready libraries  
**Hands-on time:** Less than 30 mins  
**Walk-away time:** 3 hours  
**Throughput:** Flexible, 4 to 24 rxns in a single run  
**Chemistry:** DNA EF Library Prep (powered by Watchmaker)

## Highly reproducible output library size across input sources and masses

Callisto generates libraries that are suitable for whole genome sequencing. The library prep App consistently delivers mode library size of approximately 600 bp across a broad input DNA mass range from 100ng-500ng.

## Compatible with wide input DNA range

Callisto generates library concentrations exceeding 2 nM(with qPCR) with input DNA amounts as low as 100 ng. The system also demonstrates compatibility with higher input amounts, supporting DNA masses up to 500 ng, ensuring flexibility across a broad range of sample inputs.

## **Equivalent yield from various DNA sources**

The App is compatible with various input DNA sources yielding about the same concentration of libraries across the DNA types. The output concentrations exceed 2 nM in all cases. The DNA sources include two human reference genomes (HG001, HG002), bacterial DNA (ZymoBIOMICS Microbial Community Standard), and DNA extracted from whole blood (WB).

## Robust variant calling performance

Callisto-prepped libraries deliver highly accurate variant calling results across multiple technical replicates (n=5) in SNP and INDEL precision, recall, and F1 scores. Libraries were prepared using HG002 reference DNA and sequenced on Illumina NovaSeq X. Variant calling was performed with NVIDIA Parabricks 4.4.0.1 and benchmarked against the most recent NIST benchmark (HG002_GRCh38_1_22_v4.2.1_benchmark_noinconsistent.bed) using hap.py v0.3.12.

To process samples using DNA Library Prep for Illumina you will need the following materials:

- Volta Labs  
  Callisto Complete Kit for DNA EF Library Prep (powered by Watchmaker) - 96 rxns  
  LP0002  
  Callisto Complete Kit for DNA EF Library Prep (powered by Watchmaker) - 480 rxns, HT  
  LP0004  
- Beckman Coulter*  
  SPRIselect Beads  
  B23319  
- Twist Biosciences*#  
  Twist Full Length Unique Dual Index (UDI) Adapters  
  Twist offers 16 adapter plates. Example: plate 1 (107376)  
- IDT DNA*#  
  xGen™ UDI-UMI Adapters, 96 rxn  
  10005903

*Please contact our partners directly to purchase these reagents.

#You only need one of these adapters, not both.

To order: sales@voltalabs.co