## Miniaturized Library Preps with High Yields and Sequencing Data

Volta’s ONT LSK114 DNA Library Prep App offers an automated, streamlined process to convert high molecular weight DNA into Oxford Nanopore-compatible, sequencing-ready libraries at a miniaturized reaction scale. This App supports whole-genome sequencing, targeted sequencing, and metagenomics applications.

### Key Benefits

- **Maximized output:** Achieve >90 Gb on PromethION from a single library load over 72 hours.
- **Lower input requirements:** 2x higher library yield enables the same output with less DNA input.
- **Reduced costs & time:** Cut prep costs via 2x miniaturization and up to 6x less hands-on time.

## App Specifications

**Input:** 1 μg, or 100-200 fmol of high molecular weight DNA  
**Output:** Sequencing ready libraries  
**Hands-on time:** Less than 30 minutes  
**Walk-away time:** 3.5 hours  
**Throughput:** Flexible, 4 - 24 rxns in a single run  
**Chemistry:** Ligation Sequencing Kit V14 + NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing

Callisto generates significantly higher library yields across a wide range of DNA input sizes compared to manual preps. 1 µg of input DNA yields enough library to load a PromethION flowcell and generate high quality sequencing data.

## Libraries Retain Integrity of Input DNA

The App uses a gentle process that prevents shearing, preserving the integrity of high molecular weight input DNA in the output libraries and enabling high N50 values when sequenced. As shown in the figure, the size distribution of input DNA and output libraries demonstrates strong overlap with only minimal shift.

## High Quality Sequencing on Oxford Nanopore Sequencing Platforms

Libraries prepared on Callisto deliver high-quality sequencing results on MinION and PromethION platforms with exceptional scores on key metrics including N50, Q score, and pore occupancy with single loading. Consistently generating >90 GB of data is now feasible on PromethION flowcell due to the high efficiency library preparation process. The figure shows data obtained on a PromethION flowcell that was loaded once with 400 ng of the libraries prepared on Callisto.

To process samples using DNA Library Prep for Oxford nanopore LSK114 you will need the following materials:

- **Volta Labs**
  - DNA Library Prep for ONT (LSK114) - 48 rxns, Accessory Kit
  - DNA Library Prep for ONT (LSK114) - 480 rxns, HT Accessory Kit  
- **Ligation Sequencing Kit V14**
  - SQK-LSK114
  - SQK-LSK114-XL
- **NEBNext® Companion Module v2 for Oxford Nanopore Technologies® Ligation Sequencing**  
  - E7672S  
  - E7672L

*Please contact suppliers directly to purchase these reagents.*

**To order:** sales@voltalabs.co

**Data sheet**  
**DNA Library Prep for Oxford Nanopore LSK114**

[Scaling Oxford Nanopore Sequencing: VoltaPrep for LSK114 Brings Automation to Applied Genomics](https://www.voltalabs.com/post/scaling-oxford-nanopore-sequencing-voltaprep-for-lsk114-brings-automation-to-applied-genomics)