## BEST-IN-CLASS ENRICHMENT, SIMPLIFIED FOR EVERY LAB

The Twist Standard Hyb v2 App on Callisto delivers high-performance hybridization capture using Twist Bioscience's trusted chemistry—fully automated from input libraries to enriched, PCR ready libraries.

Harness the power of Twist’s hybrid capture chemistry with Callisto's walk-away platform for unmatched performance and simplicity.

### Key Benefits

- **Consistent Performance:** Callisto handles hybridization, heated washes with reproducible enrichment results.
- **Uniform Coverage:** Ensure balanced enrichment across all GC regions.
- **Simpler Workflow:** A simpler capture workflow that anyone in the lab can run.

## App Specifications

**Input:**  500 ng - 1.5 μg DNA libraries  
**Output:** Enriched libraries ready for PCR  
**Hands-on time:** Less than 30 minutes  
**Callisto processing time:** 18 hours  
**Throughput:** 4 - 24 rxns in a single run  
**Multiplexing:** Flexible, 1 - 8 libraries  
**Panel Size:**  0.2 MB to 36 MB  
**Chemistry:** Twist Standard Hybridization Kit v2

## High Quality Sequencing Metrics

Callisto delivers high uniformity and on-target rates comparable or better than the manual workflow. The data presented here is using the Twist Exome 2.0 panel downsampled to 50 M reads.

## Uniform Coverage Across Targets

Callisto delivers unbiased coverage across a wide range of GC contents on par with manually processed samples. The data presented here uses the Twist Exome 2.0 panel.

## Excellent Target Coverage for Difficult to Capture Regions (ACTA1 and RASA4B Exon)

ACTA1 and RASA4B are select examples of difficult to capture genes that are systematically undercovered. Hybridization capture on Callisto yields high depth of coverage in these gene regions of interest, with minimal or no dropouts for both high GC and low GC regions.

To process samples using _Hybridization Capture for Twist (Standard Hyb v2)_ you will need the following materials:

- **Volta Labs Hybridization Capture for Twist (Standard v2) - 12 rxns Accessory Kit**  
  HC0004  
- **Twist Bioscience**  
  Twist Standard Hyb and Wash Kit v2 with Amp Mix, 96 rxn  
  105561  
- **Twist Universal Blockers, 96 rxn**  
  100767  
- **Twist Binding and Purification Beads, 96 rxn**  
  100984  
- **Twist Hybridization Capture Panels**  
  N/A  
  
*Please contact Twist Bioscience directly to purchase these reagents.*  
Simply contact sales@twistbioscience.com for more information, or learn more at [twistbioscience.com/products/ngs](https://www.twistbioscience.com/products/ngs).