## Simplify workflow with walk-away, end-to-end hybridization capture

Volta's IDT xGen™ v2 Hybridization Capture offers fully automated hybridization, capture, washes, and PCR setup to deliver high-quality libraries without inter-operator variability. Allows you to process up to 480 exomes per run.

### Key benefits

- Decrease your sequencing redo rates to less than 2%
- Achieve uniform coverage across high- and low-GC content regions
- Save on probe costs by plexing up to 20 libraries

## App Specifications

Input: 500 ng to 10µg of DNA libraries  
**Output:** Amplification libraries ready to sequence  
**Hands-on time:** Less than 30 mins  
**Callisto processing time:** 21 hours  
**Throughput:** Flexible, 4 to 24 rxns in a single run  
**Multiplexing:** Flexible, 1-20 libraries  
**Panel Size:** ~10k (Pan-Cancer) to ~400k (Exome) probes  
**Chemistry:** IDT xGen™ Hybridization Capture (HC0001)

## Uniform on-target coverage independent of operator

Callisto delivers high uniformity and on-target rates. This maximizes use of sequencing reads for better coverage of target regions, reducing the need for excessive sequencing depth and lowering overall costs.

## Plexing with up to 20 libraries without loss in uniformity

Pooling more libraries into a single hybridization capture reaction on Callisto allows for greater throughput without compromising on uniformity and on-target percentages. Up to 480 libraries can be processed simultaneously using an exome panel. Higher throughput in combination with significantly reduced hands-on time leads to considerable cost savings.

## Excellent target coverage for difficult to capture regions (RB1 and TERT exon)

RB1 and TERT are difficult to capture cancer-associated genes due to their high GC content and repetitive sequences. Hybridization capture on Callisto yields higher than 90% on-target percentages with high depth of coverage in these two gene regions of interest, with minimal or no dropouts for both high GC and low GC regions.

## Accurate detection of low frequency variants with Callisto over manual preps

The EGFR rs121434568 C>T SNP occurs at a low expected frequency of 1% in the Horizon cfDNA reference sample. Hybridization capture on Callisto accurately identified the C>T variant at the expected frequency of 1%.

To process samples using Hyb Capture for IDT xGEN App, you will need the following materials:

- Volta Labs Hybridization Capture for IDT (xGen™ v2) - 16 rxns Accessory Kit  
- HC0001 IDT DNA*  
- xGen Hybridization and Wash v2 Reagents, 16 reactions kit  
- 10010351  
- xGen Hybridization and Wash v2 Beads, 16 reactions  
- Streptavidin Beads  
- 10010353  
- xGen™ Predesigned Hybridization Capture Panels  
- NA  
- xGen Universal Blockers TS, 16 reactions  
- 1075474  
- xGen Library Amplification Primer Mix, 16 reactions  
- 1077675  
- Beckman Coulter* AMPure XP beads  
- A63880

\*Please contact our partners directly to purchase these reagents.