# High-quality WGS starts with better sample prep.

Callisto™ delivers fully automated, pre-validated workflows for whole genome sequencing—so your lab can scale with confidence, consistency, and the quality standards needed for clinical use.

## Say goodbye to tedious clinical WGS prep and unpredictable outcomes.

### Why choose Callisto:

With Callisto, every run delivers the same high-quality libraries—regardless of operator skill level—thanks to pre-validated workflows, precision liquid handling, and tightly controlled processing conditions. This unmatched consistency means fewer repeats, cleaner data, and greater confidence in every result.

## WHY CALLISTO FOR WGS

### Consistently Superior Results

Callisto delivers highly uniform fragment sizes, and cleaner libraries for every batch. Designed to meet the rigid standards of clinical‑grade sequencing.

### Fast Setup, Minimal Oversight

Achieve full setup in just 30 minutes, with 3 hours of hands-free runtime—allowing your team to focus on higher-value tasks without sacrificing throughput.

### Turnkey and Ready on Day One

Pre-loaded and pre-validated WGS workflows let you start sample preparation immediately—no method development, optimization, or costly trial-and-error. Callisto gets you from first run to production faster, accelerating your path to high-quality data and results. Focus on discoveries, not delays.

### Effortless Operation with No Expertise Needed

A clean, intuitive interface and guided workflow mean even users with minimal lab experience can run complex WGS preparations. Push-button simplicity—load your samples, select your app, and walk away.

## BROAD NGS COMPATIBILITY

Callisto also offers broad NGS compatibility for both long- and short-read sequencing—making it ideal for expanding your test menu without costly new equipment and method development investments.

## Expanded Chemistry Compatibility

Callisto™ now supports library-preparation workflows for a wide range of leading whole-genome sequencing chemistries — including reagents from Illumina, New England Biolabs (NEB), Twist Bioscience, KAPA/Roche, Qiagen, and Watchmaker Genomics — all validated for use with multiple sequencers. Laboratories such as GeneDx are already utilizing Callisto’s chemistry-agnostic platform to streamline high-quality WGS operations across diverse reagent systems.

With this expanded flexibility, your lab can choose the chemistry AND sequencer that best match your workflow — without being locked into one system.

## Built to Flex with Your Platform

Callisto delivers validated, high-quality library prep across all major sequencing platforms and chemistries—Illumina, Twist, NEB, KAPA, Qiagen, and Watchmaker. Whether you’re scaling clinical WGS testing or ensuring reproducible results across batches, Callisto gives you complete flexibility without compromising performance.

## TRUSTED BY LEADERS IN GENOMICS

Hartwig Medical Foundation: Co-developing applications for cfDNA, tissue, and long-read sequencing workflows

Callisto already delivers an excellent hybrid capture workflow… we are now working together to enable whole genome sequencing on various DNA sources…”

Ewart de Bruijn

Hartwig Medical Foundation

UMC Utrecht: Integrating Callisto into routine clinical WGS workflows

Callisto's walk-away automation, powered by advanced digital fluidics, is truly a game-changer. It allows our clinical technicians to simply load 24 DNA samples and produce clinical-grade sequencing-ready libraries seamlessly in <3 hours.”

Marcel Nelen

UMC Utrecht

## CALLISTO VS. TRADITIONAL PREP

| Feature                                    | Callisto                     | Manual                         |
|--------------------------------------------|------------------------------|--------------------------------|
| Liquid Handler                             |                              |                                |
| Hands-on time                              | <30 min                      | 3–5 hrs                       |
| Operator walk away time                    | True uninterrupted walk-away automation – load and go | None – requires constant hands-on work |  
| Throughput (libraries/day)                | 72 Illumina                  | 8–12                          |
| Cross-platform sequencing compatibility     | Fully compatible, no reprogramming needed | Protocol specific training required |

## READY TO MAKE CLINICAL WHOLE GENOME SEQUENCING LIBRARY PREP EASIER?

WEBINAR

WGS Without Limits:

Powering Clinical Genetics at UMC Utrecht with Callisto

**DATE:** Tuesday, 7 October 2025

**TIME:** 7AM PT | 10AM ET | 4PM CEST